Immunoelectron microscopy pinpoints glutathione-peroxidase (GSH-PO) in neonatal rat adrenal cortical cells.
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چکیده
منابع مشابه
Immunolocalization of Glutathione-Peroxidase (GPx1) in the Rat Adrenal Cortex: Correlation between Steroidogenesis and Lipid Peroxidation
In order to confirm the relationship between glutathione-peroxidase (GPx1) and biological significance on steroidogenesis, we have studied the immunocytochemical localization of GPx1 in the rat adrenal cortical cells. GPx1 was observed not only in cytoplasm (cytosol GPx1) but in mitochondria (mitochondrial GPx1). The staining intensity was altered by the functional state of the adrenal cortical...
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Immunolocalization of antigens in cells requires simultaneous preservation of structure and antigenicity. The difficulties of immunolocalization methods are centered around the methods used to preserve structure, which as a rule tend to degrade the ability of antigens (usually proteins) to be recognized by their cognate antibodies. The value of immunolocalization for cell biology was early reco...
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Introduction Reduced glutathione, most commonly called glutathione or GSH, is a relatively small molecule ubiquitous in living systems. Occurring naturally in all human cells, GSH is a water-phase orthomolecule. Its intracellular depletion ultimately results in cell death and its clinical relevance has been researched for decades. GSH is the smallest intracellular thiol (–SH) molecule. Its high...
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Calmodulin, a multifunctional Ca(++)-binding protein, is present in all eucaryotic cells. We have investigated the distribution of this protein in the rat cerebellum by immunoelectron microscopy using a Fab-peroxidase conjugate technique. In Purkinje and granular cell bodies, calmodulin reaction product was found localized both on free ribosomes and on those attached to rough endoplasmic reticu...
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ژورنال
عنوان ژورنال: Cell Structure and Function
سال: 1987
ISSN: 0386-7196,1347-3700
DOI: 10.1247/csf.12.181